Participating Faculty

James Pru

James Pru

Department:Animal Sciences & Associate Faculty in SMB; WSU
Credentials:2000~Ph.D. University of Wyoming
Office:Animal Sciences VMRB171
Mailing Address:Animal Sciences
PO Box 646310
Pullman, WA 99164-6310

Research Interests

Understanding the molecular dialog of early pregnancy, mechanisms of endometrial regeneration, alternative progesterone signaling

Research Summary

My research focuses on understanding molecular events coordinating the physiology of embryo implantation using the mouse and domestic ruminants as model organisms. With a basic understanding of these processes, the long-term goal is to identify mechanisms that, when gone awry, contribute to infertility during early gestation. Related to embryo implantation, we are specifically interested in the processes by which the embryo signals its presence as it invades the uterine wall in an effort to establish an exchange apparatus for nutrients and waste. As progesterone coordinates many of the uterine events during embryo implantation, we are also interested in how ovarian-derived progesterone signals in the endometrium by non-classical mechanisms. We recently demonstrated expression of a novel membrane progestin receptor in the uterus and ongoing studies are designed to test the functional importance of this putative receptor in uterine physiology. In related studies, efforts are being made to learn how resident stem/progenitor cells of the endometrium contribute to uterine repair during the estrous/menstrual cycle and following parturition.

Research Publications


McCallum ML, Pru CA, Niikura Y, Yee SP, Lydon JP, Peluso JJ, Pru JK. Conditional ablation of progesterone receptor membrane component 1 results in subfertility in the female and development of endometrial cysts. Endocrinology 2016;157;3309-3319. PMID: 27309940

Clark NC, Friel AM, Pru CA, Zhang L, Shioda T, Rueda BR, Peluso JJ, Pru JK. Progesterone receptor membrane component 1 promotes survival of human breast cancer cells and growth of xenograft tumors. Cancer Biol Ther 2016;17:262-271. PMID:26785864

Dey S, Chamero P, Pru JK, Chien MS, Ibarra-Soria X, Spencer KR, Logan DW, Matsunami H, Peluso JJ, Stowers L. Cyclic regulation of sensory perception by a female hormone alters behavior. Cell 2015;161:1334-1344. PMID: 26046438 (

Henkes LE, Pru JK, Ashley RL, Anthony RV, Veeramachaneni DNR, Gates KC, Hansen TR. Embryo mortality in Isg15-/- mice is exacerbated by environmental stress. Biol Reprod 2015;92:36. PMID: 25505199

*Friel AM, Zhang L, Pru CA, Clark NC, McCallum ML, Blok LJ, Shioda T, Peluso JJ, Rueda BR, Pru JK. Progesterone receptor membrane component 1 deficiency attenuates growth while promoting chemosensitivity of human endometrial xenograft tumors. Cancer Lett 2015;356:434-442. PMID:25304370 (

Hansen TR, Pru JK. ISGylation: A conserved pathway in mammalian pregnancy. Adv Exp Med Biol 2014; 759:13-31. PMID: 25030758

Griffin D, Liu X, Pru CA, Pru JK, Peluso JJ. Expression of progesterone receptor membrane component-2 within the immature rat ovary and its role in regulating mitosis and apoptosis of spontaneously immortalized granulosa cells. Biol Reprod 2014;91:36. PMID: 24990806

Peluso JJ, Pru JK. Non-canonical progesterone signaling in granulosa cell function. Reproduction 2014;147:R169-178. PMID: 24516175

Filant J., DeMayo FJ, Pru JK, Lydon JP, Spencer TE. Fibroblast growth factor receptor two (FGRF2) regulates uterine epithelial integrity and fertility in mice. Biol Reprod. 2014;90:1-11. PMID: 24227756

*Pru JK, Clark NC. PGRMC1 and PGRMC2 in uterine physiology and disease. Front Neurosci. 2013; 7:168. PMID: 24065879

Washington State University